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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ARHGEF19 Recombinant monoclonal antibody (82966-2-RR)

    IF Staining of U2OS using 82966-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ARHGEF19 antibody (82966-2-RR, Clone: 230290F5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of U-2 OS using ARHGEF19 Recombinant monoclonal antibody (82966-2-RR)

    FC experiment of U-2 OS using 82966-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of HeLa cells using ARHGEF19 Recombinant monoclonal antibody (82966-2-RR)

    FC experiment of HeLa using 82966-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82966-2-RR

    Affinity and Kinetic Characterization of 82966-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82966-2-RR against Human ARHGEF19 were performed. The affinity constant is below 1 pM.

ARHGEF19 Recombinant monoclonal antibody

ARHGEF19 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82966-2-RR
Clone No.230290F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

230290F5, Ephexin-2, WGEF

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82966-2-RR targets ARHGEF19 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11645

Product name: Recombinant human ARHGEF19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 454-802 aa of BC040640

Sequence: VVLDHCPAFRRVYLPYVTNQAYQERTYQRLLLENPRFPGILARLEESPVCQRLPLTSFLILPFQRITRLKMLVENILKRTAQGSEDEDMATKAFNALKELVQECNASVQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKLKLSSKAVYLHLFNDCLLLSRRKELGKFAVFVHAKMAELQVRDLSLKLQGIPGHVFLLQLLHGQHMKHQFLLRARTESEKQRWISALCPSSPQEDKEVISEGEDCPQVQCVRTYKALHPDELTLEKTDILSVRTWTSDGWLEGVRLADGEKGWVPQAYVEEISSLSARLRNLRENKRVTSATSKLGEAPV

相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 19
Calculated molecular weight 802 aa, 89 kDa
GenBank accession numberBC040640
Gene Symbol ARHGEF19
Gene ID (NCBI) 128272
RRIDAB_3670712
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8IW93
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rho guanine nucleotide exchange factors (RHOGEFs) are proteins that could regulate Rho GTPases, which can be involved in a variety of physiological processes, such as survival, development, differentiation, and motility. ARHGEF19 also known as WGEF, is a member of the RhoGEF family. ARHGEF19 contains two identical functional domains, including the Dbl homology (DH) and pleckstrin homology (PH) domain(PMID:32993957). ARHGEF19 was highly expressed in human breast cancer(PMID: 34813497).

Protocols

Product Specific Protocols
IF protocol for ARHGEF19 antibody 82966-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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