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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    WB analysis of mouse testis using 11243-1-AP (same clone as 11243-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11243-1-AP (ARHGEF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human prostate cancer using 11243-1-AP (same clone as 11243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of mouse kidney using 11243-1-AP (same clone as 11243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of mouse kidney using 11243-1-AP (same clone as 11243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human prostate cancer using 11243-1-AP (same clone as 11243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human kidney using 11243-1-AP (same clone as 11243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11243-1-AP (ARHGEF18 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IHC staining of human kidney using 11243-1-AP (same clone as 11243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11243-1-AP (ARHGEF18 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARHGEF18 Polyclonal antibody (11243-1-AP)

    IF Staining of A431 using 11243-1-AP (same clone as 11243-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARHGEF18 antibody (11243-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11243-1-PBS in a different storage buffer formulation.

ARHGEF18 Polyclonal antibody, PBS Only

ARHGEF18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11243-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

114 kDa Rho-specific guanine nucleotide exchange factor, KIAA0521, p114 Rho GEF, P114 RhoGEF, p114RhoGEF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11243-1-PBS targets ARHGEF18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1749

Product name: Recombinant human ARHGEF18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-89 aa of BC014994

Sequence: MSQGMQRMHLETLQQVDKWPLCGPLACSELLQLTVRSLEGWRKEVLGSIKGAGTSQGGEIHPRSSSGGERAHVKPCSAPQALVVGLGPG

相同性解析による交差性が予測される生物種
Full Name rho/rac guanine nucleotide exchange factor (GEF) 18
Calculated molecular weight 131 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC014994
Gene Symbol ARHGEF18
Gene ID (NCBI) 23370
RRIDAB_2877753
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZSZ5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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