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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGEF11 Polyclonal antibody (30508-1-AP)

    WB analysis using 30508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30508-1-AP (ARHGEF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ARHGEF11 Polyclonal antibody (30508-1-AP)

    IHC staining of mouse colon using 30508-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30508-1-AP (ARHGEF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ARHGEF11 Polyclonal antibody

ARHGEF11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30508-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

ARHGEF11, DKFZp667F1223, GTRAP48, KIAA0380, PDZ RHOGEF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HuH-7 cells, MDA-MB-453 cells, mouse brain tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30508-1-AP targets ARHGEF11 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF11 fusion protein Ag33104 相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 11
Calculated molecular weight168 kDa
Observed molecular weight200-220 kDa
Gene Symbol ARHGEF11
Gene ID (NCBI) 9826
RRIDAB_3086341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).

Protocols

Product Specific Protocols
WB protocol for ARHGEF11 antibody 30508-1-APDownload protocol
IHC protocol for ARHGEF11 antibody 30508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols