Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 11363-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11363-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11363-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11363-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11363-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 11363-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11363-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 11363-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 11363-1-AP
IP result of anti-ARHGEF1 (IP:11363-1-AP, 4ug; Detection:11363-1-AP 1:1000) with HEK-293 cells lysate 2800ug.
IHC staining of human colon using 11363-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 11363-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 11363-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARHGEF1 antibody (11363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 11363-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ARHGEF1 Polyclonal antibody

ARHGEF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11363-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

p115-RhoGEF, p115RhoGEF, P115 RHOGEF, LSC, LBCL2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, human spleen tissue, Jurkat cells, K-562 cells, mouse spleen tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
CoIP	See 1 publications below

Product Information

11363-1-AP targets ARHGEF1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1905 Product name: Recombinant human ARHGEF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC011726 Sequence: MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEP 相同性解析による交差性が予測される生物種
Full Name	Rho guanine nucleotide exchange factor (GEF) 1
Calculated molecular weight	912 aa, 102 kDa
Observed molecular weight	102-110 kDa
GenBank accession number	BC011726
Gene Symbol	ARHGEF1
Gene ID (NCBI)	9138
RRID	AB_2059741
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92888
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for ARHGEF1 antibody 11363-1-AP	Download protocol
IHC protocol for ARHGEF1 antibody 11363-1-AP	Download protocol
IP protocol for ARHGEF1 antibody 11363-1-AP	Download protocol
WB protocol for ARHGEF1 antibody 11363-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Authors - Silvana Konermann View Article
human	WB	Nat Metab Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression Authors - Lin Qian View Article
human	WB	Mol Biol Cell The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα. Authors - P V Ryder View Article
mouse	CoIP,WB	Nat Commun Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis Authors - Yaping Zhang View Article
human	WB	Cancer Lett Apigenin inhibits tumor angiogenesis by hindering microvesicle biogenesis via ARHGEF1 Authors - Wanying Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 11363-1-AP

WB analysis of HepG2 using 11363-1-AP

WB analysis of Jurkat using 11363-1-AP

WB analysis of HeLa using 11363-1-AP

WB analysis of K-562 using 11363-1-AP

WB analysis of human spleen using 11363-1-AP

WB analysis of human brain using 11363-1-AP

WB analysis of mouse spleen using 11363-1-AP

IP experiment of HEK-293 using 11363-1-AP

IHC staining of human colon using 11363-1-AP

IHC staining of human colon using 11363-1-AP

IF Staining of A549 using 11363-1-AP

IF Staining of A549 using 11363-1-AP

ARHGEF1 Polyclonal antibody

ARHGEF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression

The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.

Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis

Apigenin inhibits tumor angiogenesis by hindering microvesicle biogenesis via ARHGEF1