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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGAP9 Polyclonal antibody

ARHGAP9 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15665-1-AP

Synonyms

10C, ARHGAP9, RGL1

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 15665-1-AP

    WB analysis of Jurkat using 15665-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 15665-1-AP

    WB analysis of HL-60 using 15665-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15665-1-AP

    WB analysis of HeLa using 15665-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15665-1-AP

    IF Staining of HeLa using 15665-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15665-1-AP (ARHGAP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15665-1-AP targets ARHGAP9 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP9 fusion protein Ag8104 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 9
Calculated molecular weight 750 aa, 83 kDa
Observed molecular weight 70 kDa, 65 kDa
GenBank accession numberBC006107
Gene symbol ARHGAP9
Gene ID (NCBI) 64333
RRIDAB_2059566
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.

Protocols

Product Specific Protocols
WB protocol for ARHGAP9 antibody 15665-1-APDownload protocol
IF protocol for ARHGAP9 antibody 15665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genomics

ARHGAP9 knockdown promotes lung adenocarcinoma metastasis by activating Wnt/β-catenin signaling pathway via suppressing DKK2

Authors - Wenping Song
View Article