Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HL-60 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15665-1-AP targets ARHGAP9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8104 Product name: Recombinant human ARHGAP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 402-750 aa of BC006107 Sequence: VLHIRTIPGHEFLLQSDHETELRAWHRALRTVIERLVRWVEARREAPTGRDQGSGDRENPLELRLSGSGPAELSAGEDEEEESELVSKPLLRLSSRRSSIRGPEGTEQNRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFLRLCIAAVDKRGLDVDGIYRVSGNLAVVQKLRFLVDRERAVTSDGRYVFPEQPGQEGRLDLDSTEWDDIHVVTGALKLFLRELPQPLVPPLLLPHFRAALALSESEQCLSQIQELIGSMPKPNHDTLRYLLEHLCRVIAHSDKNRMTPHNLGIVFGPTLFRPEQETSDPAAHALYPGQLVQLMLTNFTSLFP 相同性解析による交差性が予測される生物種 |
| Full Name | Rho GTPase activating protein 9 |
| Calculated molecular weight | 750 aa, 83 kDa |
| Observed molecular weight | 70 kDa, 65 kDa |
| GenBank accession number | BC006107 |
| Gene Symbol | ARHGAP9 |
| Gene ID (NCBI) | 64333 |
| RRID | AB_2059566 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRR9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARHGAP9 antibody 15665-1-AP | Download protocol |
| WB protocol for ARHGAP9 antibody 15665-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
