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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP4 Recombinant antibody (85648-2-RR)

    WB analysis using 85648-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85648-2-RR (ARHGAP4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ARHGAP4 Recombinant antibody (85648-2-RR)

    IF Staining of Jurkat using 85648-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (85648-2-RR, Clone: 242995B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85648-2-RR

    Affinity and Kinetic Characterization of 85648-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85648-2-RR against Human ARHGAP4 were performed. The affinity constant is 0.518 nM.

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ARHGAP4 Recombinant antibody

ARHGAP4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85648-2-RR
Clone No.242995B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85648-2-RR targets ARHGAP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARHGAP4 fusion protein Ag10091 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 4
Calculated molecular weight 986 aa, 109 kDa
Observed molecular weight 115-120 kDa
GenBank accession numberBC052303
Gene Symbol ARHGAP4
Gene ID (NCBI) 393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP98171
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGAP4 (Rho GTPase-Activating Protein 4), also named as KIAA0131, RGC1, RHOGAP4, is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating these signaling molecules. Rho GTPases are critical regulators of cytoskeletal dynamics, cell motility, and intracellular trafficking.

Protocols

Product Specific Protocols
WB protocol for ARHGAP4 antibody 85648-2-RRDownload protocol
IF protocol for ARHGAP4 antibody 85648-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols