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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGAP29 Polyclonal antibody

ARHGAP29 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12583-1-AP

Synonyms

ARHGAP29, PARG1, RP11 255E17.1

Validation Data Gallery

Applications:
  • WB analysis using 12583-1-AP

    WB analysis using 12583-1-AP

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 12583-1-AP (ARHGAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12583-1-AP

    IHC staining of human colon cancer using 12583-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12583-1-AP (ARHGAP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP29 antibody included
  • IHC staining of human breast cancer using 12583-1-AP

    IHC staining of human breast cancer using 12583-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12583-1-AP (ARHGAP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARHGAP29 antibody included

Tested Applications

Positive WB detected inHCT 116 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARHGAP29 Ready-To-Use IHC Kit. ARHGAP29 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12583-1-AP targets ARHGAP29 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP29 fusion protein Ag3282 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 29
Calculated molecular weight 142 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC022483
Gene symbol ARHGAP29
Gene ID (NCBI) 9411
RRIDAB_2918030
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP29 antibody 12583-1-APDownload protocol
IHC protocol for ARHGAP29 antibody 12583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of Radil as a Ras binding partner and putative activator.

Authors - Byeong Hyeok Choi
View Article
humanWB

Hepatol Res

circTMEM181 upregulates ARHGAP29 to inhibit hepatocellular carcinoma migration and invasion by sponging miR-519a-5p

Authors - Bing Chen
View Article