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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP28 Polyclonal antibody (26766-1-AP)

    WB analysis using 26766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26766-1-AP (ARHGAP28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARHGAP28 Polyclonal antibody (26766-1-AP)

    IHC staining of mouse testis using 26766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26766-1-AP (ARHGAP28 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARHGAP28 Polyclonal antibody (26766-1-AP)

    IHC staining of mouse testis using 26766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26766-1-AP (ARHGAP28 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ARHGAP28 Polyclonal antibody

ARHGAP28 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26766-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1314, cDNA FLJ58638, highly similar to Homo sapiens Rho GTPase activating protein 28

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC2C12 cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26766-1-AP targets ARHGAP28 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP28 fusion protein Ag25293 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 28
Observed molecular weight 82-90kDa
GenBank accession numberBC065274
Gene Symbol ARHGAP28
Gene ID (NCBI) 79822
RRIDAB_3669567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDB4DXL2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGAP28 (Rho GTPase Activating Protein 28), also known as FLJ10312 and KIAA1314, is a Rho GTPase activating protein (RhoGAP) that switches RhoA to its inactive form. It is reported that high expression of ARHGAP28 in osteosarcoma cell lines can inhibit the proliferation, migration, and invasion of osteosarcoma(PMID: 38041020).

Protocols

Product Specific Protocols
WB protocol for ARHGAP28 antibody 26766-1-APDownload protocol
IHC protocol for ARHGAP28 antibody 26766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Exp Pharmacol Physiol

ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.

Authors - Li Wang
View Article