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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ARHGAP25 Polyclonal antibody

ARHGAP25 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14349-1-AP

Synonyms

ARHGAP25, KAIA0053, KIAA0053

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 14349-1-AP

    WB analysis of Jurkat using 14349-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14349-1-AP (ARHGAP25 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 14349-1-AP

    WB analysis of Jurkat using 14349-1-AP

    WB result of ARHGAP25 antibody (14349-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP25 transfected Jurkat cells.

  • IP experiment of Jurkat using 14349-1-AP

    IP experiment of Jurkat using 14349-1-AP

    IP result of anti-ARHGAP25 (IP:14349-1-AP, 4ug; Detection:14349-1-AP 1:500) with Jurkat cells lysate 1000ug.

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human tonsillitis using 14349-1-AP

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14349-1-AP targets ARHGAP25 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP25 fusion protein Ag5691 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 25
Calculated molecular weight 73 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC039591
Gene symbol ARHGAP25
Gene ID (NCBI) 9938
RRIDAB_2059273
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IHC protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IP protocol for ARHGAP25 antibody 14349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop

Authors - Sijia Han
  • KD Validated
View Article