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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP21 Polyclonal antibody (22183-1-AP)

    WB analysis using 22183-1-AP (same clone as 22183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ARHGAP21 Polyclonal antibody (22183-1-AP)

    IHC staining of human ovary cancer using 22183-1-AP (same clone as 22183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ARHGAP21 Polyclonal antibody (22183-1-AP)

    IHC staining of human prostate cancer using 22183-1-AP (same clone as 22183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ARHGAP21 Polyclonal antibody (22183-1-AP)

    IHC staining of human prostate cancer using 22183-1-AP (same clone as 22183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARHGAP21 Polyclonal antibody (22183-1-AP)

    IF Staining of HepG2 using 22183-1-AP (same clone as 22183-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARHGAP21 antibody (22183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22183-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARHGAP21 Polyclonal antibody, PBS Only

ARHGAP21 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22183-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Rho-type GTPase-activating protein 21, Rho GTPase-activating protein 10, KIAA1424, ARHGAP10

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22183-1-PBS targets ARHGAP21 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16515

Product name: Recombinant human ARHGAP21 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1598-1957 aa of BC118915

Sequence: LDSSRLSPEVQSVAESKGDEADDERSELISEGRPVETDSESEFPVFPTALTSERLFRGKLQEVTKSSRRNSEGSELSCTEGSLTSSLDSRRQLFSSHKLIECDTLSRKKSARFKSDSGSLGDAKNEKEAPSLTKVFDVMKKGKSTGSLLTPTRGESEKQEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGGHRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSAQDLSISDWLARERLRTSTSDLSRGEIGDPQTENPSTREIATTDTPLSLHCNTGSSSSTLASTNRPLLSIPPQSPDQINGESFQNVSKNASSAANAQPHKLSETPGTKAEFHPCL

相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 21
Calculated molecular weight 1957 aa, 217 kDa
Observed molecular weight 250 kDa
GenBank accession numberBC118915
Gene Symbol ARHGAP21
Gene ID (NCBI) 57584
RRIDAB_2879018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T5U3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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