Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MDA-MB-453s cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28409-1-AP targets ARHGAP18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29144 Product name: Recombinant human ARHGAP18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 175-399 aa of BC107416 Sequence: FAQQRESKETAPGGTESQSLRTNENKYQGRDDEASNLVGEEKLIPPEETPAPETDINLEVSFAEQALNQKESSKEKIQKSKGDDATLPSFRLPKDKTGTTRIGDLAPQDMKKVCHLALIELTALYDVLGIELKQQKAVKIKTKDSGLFCVPLTALLEQDQRKVPGMRIPLIFQKLISRIEERGLETEGLLRIPGAAIRIKNLCQELEAKFYEGTFNWESVKQHDA 相同性解析による交差性が予測される生物種 |
| Full Name | Rho GTPase activating protein 18 |
| Calculated molecular weight | 663 aa, 75 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC107416 |
| Gene Symbol | ARHGAP18 |
| Gene ID (NCBI) | 93663 |
| RRID | AB_2881136 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N392 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP18 antibody 28409-1-AP | Download protocol |
| IF protocol for ARHGAP18 antibody 28409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |