Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, SK-N-SH cells, U-251 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16746-1-AP targets ARHGAP11B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10155 Product name: Recombinant human ARHGAP11B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-267 aa of BC105788 Sequence: MWDQRLVKLALLQHLRAFYGIKVKGVRGQCDRRRHETAATEIGGKIFGVPFNALPHSAVPEYGHIPSFLVDACTSLEEHIHTEGLFRKSGSVIRLKALKNKVDHGEGCLSSAPPCDIAGLLKQFFRELPEPILPADLHEALLKAQQLGTEEKNKAILLLSCLLADHTVHVLRYFFNFLRNVSLRSSENKMDSSNLAVIFAPNLLQTSEGHEKMSSNAEKKGVYQTLSWKRYQPCWVLMVSVLLHHWKALKKVNMKLLVNIREREDNV 相同性解析による交差性が予測される生物種 |
| Full Name | Rho GTPase activating protein 11B |
| Calculated molecular weight | 267 aa, 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC105788 |
| Gene Symbol | ARHGAP11B |
| Gene ID (NCBI) | 89839 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3KRB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARHGAP11B is a hominin-specific protein that promotes the development and evolutionary expansion of the brain neocortex(PMID: 33938018). ARHGAP11B arose from partial duplication of ARHGAP11A on the human lineage after separation from the chimpanzee lineage, but before the divergence from Neandertals. The antibody may cross-react with the high homology N-terminal domain of the rodent Arhgap11a, specifically with its short splicing variants, mimicking the human protein size.(PMID: 27957544)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP11B antibody 16746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |