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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGAP1 Polyclonal antibody

ARHGAP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11169-1-AP

Synonyms

ARHGAP1, CDC42GAP, p50 RhoGAP, p50rhoGAP, RHOGAP, RHOGAP1

Validation Data Gallery

Applications:
  • WB analysis of RAW 264.7 using 11169-1-AP

    WB analysis of RAW 264.7 using 11169-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 11169-1-AP

    WB analysis of PC-12 using 11169-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 11169-1-AP

    WB analysis of human heart using 11169-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human duodenum using 11169-1-AP

    WB analysis of human duodenum using 11169-1-AP

    human duodenum tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human pancreas using 11169-1-AP

    WB analysis of human pancreas using 11169-1-AP

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 11169-1-AP

    IHC staining of human pancreas using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ARHGAP1 antibody included
  • IHC staining of human pancreas using 11169-1-AP

    IHC staining of human pancreas using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ARHGAP1 antibody included
  • IHC staining of human pancreas cancer using 11169-1-AP

    IHC staining of human pancreas cancer using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ARHGAP1 antibody included
  • IF Staining of HeLa using 11169-1-AP

    IF Staining of HeLa using 11169-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRAW 264.7 cells, human duodenum tissue, human heart tissue, human pancreas tissue, PC-12 cells
Positive IHC detected inhuman pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARHGAP1 Ready-To-Use IHC Kit. ARHGAP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11169-1-AP targets ARHGAP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP1 fusion protein Ag1651 相同性解析による交差性が予測される生物種
Full Name Rho GTPase activating protein 1
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC018118
Gene symbol ARHGAP1
Gene ID (NCBI) 392
RRIDAB_2289862
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP1 antibody 11169-1-APDownload protocol
IHC protocol for ARHGAP1 antibody 11169-1-APDownload protocol
IF protocol for ARHGAP1 antibody 11169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols