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Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using Arginase-2 Polyclonal antibody (14825-1-AP)

    WB analysis of Caco-2 using 14825-1-AP (same clone as 14825-1-PBS)

    WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arginase-2 transfected Caco-2 cells. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Arginase-2 Polyclonal antibody (14825-1-AP)

    WB analysis using 14825-1-AP (same clone as 14825-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Arginase-2 Polyclonal antibody (14825-1-AP)

    WB analysis using 14825-1-AP (same clone as 14825-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14825-1-AP (Arginase-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Arginase-2 Polyclonal antibody (14825-1-AP)

    IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Arginase-2 Polyclonal antibody (14825-1-AP)

    IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Arginase-2 Polyclonal antibody (14825-1-AP)

    IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Arginase-2 Polyclonal antibody (14825-1-AP)

    IHC staining of human prostate cancer using 14825-1-AP (same clone as 14825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Arginase-2 Polyclonal antibody (14825-1-AP)

    IHC staining of human kidney using 14825-1-AP (same clone as 14825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14825-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Arginase-2 Polyclonal antibody, PBS Only

Arginase-2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14825-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ARG2, ARG 2, EC:3.5.3.1, Arginase-2, mitochondrial, arginase, type II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14825-1-PBS targets Arginase-2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6609

Product name: Recombinant human ARG2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-354 aa of BC001350

Sequence: MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI

相同性解析による交差性が予測される生物種
Full Name arginase, type II
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC001350
Gene Symbol ARG2
Gene ID (NCBI) 384
RRIDAB_2058977
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP78540
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

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