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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARFIP2 Polyclonal antibody

ARFIP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14548-1-AP

Synonyms

Arfaptin 2, ARFIP2, Partner of RAC1, POR1, Protein POR1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 14548-1-AP

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 14548-1-AP

    WB analysis of human heart using 14548-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 14548-1-AP

    WB analysis of human brain using 14548-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14548-1-AP

    WB analysis of HeLa using 14548-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 14548-1-AP

    WB analysis of mouse pancreas using 14548-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 14548-1-AP

    WB analysis of human liver using 14548-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 14548-1-AP

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 14548-1-AP

    IHC staining of human liver using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human pancreas using 14548-1-AP

    IHC staining of human pancreas using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

  • IHC staining of human pancreas using 14548-1-AP

    IHC staining of human pancreas using 14548-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

  • IF Staining of HeLa using 14548-1-AP

    IF Staining of HeLa using 14548-1-AP

    Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HepG2 using 14548-1-AP

    IF Staining of HepG2 using 14548-1-AP

    Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, human heart tissue, human liver tissue, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14548-1-AP targets ARFIP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFIP2 fusion protein Ag6076 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor interacting protein 2
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa, 45 kDa
GenBank accession numberBC000392
Gene symbol ARFIP2
Gene ID (NCBI) 23647
RRIDAB_2242930
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARFIP2 antibody 14548-1-APDownload protocol
IHC protocol for ARFIP2 antibody 14548-1-APDownload protocol
IF protocol for ARFIP2 antibody 14548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,WB,IF

J Hepatocell Carcinoma

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway

Authors - Kaida Huang
View Article