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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Arfaptin-1 Polyclonal antibody

Arfaptin-1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17726-1-AP

Synonyms

Arfaptin 1, Arfaptin-1, ARFIP1, HSU52521

Validation Data Gallery

Applications:
  • WB analysis using 17726-1-AP

    WB analysis using 17726-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17726-1-AP (Arfaptin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17726-1-AP

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of human breast cancer using 17726-1-AP

    IHC staining of human breast cancer using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17726-1-AP (ARFIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of human pancreas using 17726-1-AP

    IHC staining of human pancreas using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17726-1-AP (ARFIP1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IHC staining of human pancreas using 17726-1-AP

    IHC staining of human pancreas using 17726-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17726-1-AP (ARFIP1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with ARFIP1 antibody included
  • IF Staining of HepG2 using 17726-1-AP

    IF Staining of HepG2 using 17726-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Arfaptin-1 antibody (17726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, HCT 116 cells, HeLa cells, HepG2 cells, Jurkat cells, Raw 264.7 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARFIP1 Ready-To-Use IHC Kit. ARFIP1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

17726-1-AP targets Arfaptin-1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arfaptin-1 fusion protein Ag12122 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor interacting protein 1
Calculated molecular weight 373 aa, 42 kDa
Observed molecular weight 42 kDa, 38 kDa, 22 kDa
GenBank accession numberBC103759
Gene symbol ARFIP1
Gene ID (NCBI) 27236
RRIDAB_2227457
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IHC protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
IF protocol for Arfaptin-1 antibody 17726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang
View Article