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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of mouse brain using 15293-1-AP (same clone as 15293-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    WB analysis of Jurkat using 15293-1-AP (same clone as 15293-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15293-1-AP (ARFGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IP experiment of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    IP result of anti-ARFGAP3 (IP:15293-1-AP, 4ug; Detection:15293-1-AP 1:2000) with HepG2 cells lysate 3000 ug. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IHC staining of human breast cancer using 15293-1-AP (same clone as 15293-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARFGAP3 antibody (15293-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of A431 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of A431 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARFGAP3 Polyclonal antibody (15293-1-AP)

    IF Staining of HepG2 using 15293-1-AP (same clone as 15293-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15293-1-AP (ARFGAP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15293-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARFGAP3 Polyclonal antibody, PBS Only

ARFGAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15293-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ARFGAP1, ARF GAP 3, ADP-ribosylation factor GTPase-activating protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15293-1-PBS targets ARFGAP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7536

Product name: Recombinant human ARFGAP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC005122

Sequence: MGDPSKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDSNWSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSLASQATRKHGTDLWLDSCVVPPLSPPPKEEDFFASHVSPEVSDTAWASAIAEPSSLTSRPVETTLENNEGGQEQGPSVEGLNVPTKATLEVSSIIKKKPNQAKKGLGAKKGSLGAQKLANTCFNEIEKQAQAADKMKEQEDLAKVVSKEESIVSSLRLAYKDLEIQMKKDEKMNISGKKNVDSDRLGMGFGNCRSVISHSVTSDMQTIEQESPIMAKPRKKYNDDSD

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor GTPase activating protein 3
Calculated molecular weight 57 kDa
Observed molecular weight 57-66 kDa
GenBank accession numberBC005122
Gene Symbol ARFGAP3
Gene ID (NCBI) 26286
RRIDAB_10804035
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP61
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ARFGAP3, also named as ADP-ribosylation factor GTPase-activating protein 3, is a 516 amino acid protein, which is Widely expressed with Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate). ARFGAP3 is a GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. The calculated molecular weight of ARFGAP3 is 57 kDa, but modified ARFGAP3 is about 57-66 kDa.

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