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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HepG2 using 16519-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of mouse brain using 16519-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HeLa using 16519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HeLa using 16519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    WB analysis of HeLa using 16519-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of human ovary cancer using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of human placenta using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of mouse ovary using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of rat stomach using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of mouse kidney using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of mouse ovary using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IHC staining of rat ovary using 16519-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARFGAP2 Polyclonal antibody (16519-1-AP)

    IF Staining of HeLa using 16519-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ARFGAP2 Polyclonal antibody

ARFGAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16519-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADP-ribosylation factor GTPase-activating protein 2, ARF GAP 2, ARFGAP 2, GTPase-activating protein ZNF289, IRZ

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse brain tissue
Positive IHC detected inrat stomach tissue, mouse kidney tissue, human ovary cancer tissue, human placenta tissue, mouse ovary tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16519-1-AP targets ARFGAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9740

Product name: Recombinant human ARFGAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-344 aa of BC030148

Sequence: MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDSNWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMSSAVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKSSIIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQELQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDL

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor GTPase activating protein 2
Calculated molecular weight 521 aa, 57 kDa
Observed molecular weight 57-60 kDa
GenBank accession numberBC030148
Gene Symbol ARFGAP2
Gene ID (NCBI) 84364
RRIDAB_2878270
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for ARFGAP2 antibody 16519-1-APDownload protocol
IHC protocol for ARFGAP2 antibody 16519-1-APDownload protocol
WB protocol for ARFGAP2 antibody 16519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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