Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 10800-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10800-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10800-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10800-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10800-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10800-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10800-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 10800-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 10800-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas using 10800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human pancreas using 10800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas cancer using 10800-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10800-1-AP (ARF3 antibody) at dilution of 1:10 (under 10x lens).
IF Staining of HepG2 using 10800-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARF3 antibody (10800-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of HepG2 using 10800-1-AP
Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ARF3 Polyclonal antibody

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10800-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADP-ribosylation factor 3, ADP ribosylation factor 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, HepG2 cells, human brain tissue, mouse cerebellum tissue, Raji cells, rat brain tissue
Positive IHC detected in	human kidney tissue, human pancreas tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below

Product Information

10800-1-AP targets ARF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1174 Product name: Recombinant human ARF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC017565 Sequence: MGNIFGNLLKSLIGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLANQLKNKK 相同性解析による交差性が予測される生物種
Full Name	ADP-ribosylation factor 3
Calculated molecular weight	21 kDa
Observed molecular weight	18-20 kDa
GenBank accession number	BC017565
Gene Symbol	ARF3
Gene ID (NCBI)	377
RRID	AB_2243128
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P61204
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
IF protocol for ARF3 antibody 10800-1-AP	Download protocol
IHC protocol for ARF3 antibody 10800-1-AP	Download protocol
WB protocol for ARF3 antibody 10800-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	Development PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis Authors - Naila Umer View Article
mouse	WB	Genomics ARF3 as a novel biomarker and target in acute myeloid leukemia: Insights from pan-cancer analysis Authors - Yang Wenli View Article
human		Int J Gen Med The Improved-EFI Score: A Multi-Omics-Based Novel Efficacy Predictive Tool for Predicting the Natural Fertility of Endometriosis Patients Authors - Qiumin He View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 10800-1-AP

WB analysis of human brain using 10800-1-AP

WB analysis of Raji using 10800-1-AP

WB analysis of mouse cerebellum using 10800-1-AP

WB analysis of HeLa using 10800-1-AP

WB analysis of HepG2 using 10800-1-AP

WB analysis of rat brain using 10800-1-AP

IHC staining of human kidney using 10800-1-AP

IHC staining of human kidney using 10800-1-AP

IHC staining of human pancreas using 10800-1-AP