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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARD1A Recombinant antibody (86450-3-RR)

    WB analysis using 86450-3-RR (same clone as 86450-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86450-3-RR (ARD1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86450-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARD1A Recombinant antibody (86450-3-RR)

    IF Staining of HeLa using 86450-3-RR (same clone as 86450-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARD1A antibody (86450-3-RR, Clone: 251237F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86450-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86450-3-RR

    Affinity and Kinetic Characterization of 86450-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86450-3-RR against Human ARD1A were performed. The affinity constant is 5.33 nM.

ARD1A Recombinant monoclonal antibody, PBS Only

ARD1A Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86450-3-PBS
Clone No.251237F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARD1, EC:2.3.1.255, hARD1, NAA10, Naa10p

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86450-3-PBS targets ARD1A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6519

Product name: Recombinant human ARD1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of BC000308

Sequence: MNIRNARPEDLMNMQHCNLLCLPENYQMKYYFYHGLSWPQLSYIAEDENGKIVGYVLAKMEEDPDDVPHGHITSLAVKRSHRRLGLAQKLMDQASRAMIENFNAKYVSLHVRKSNRAALHLYSNTLNFQISEVEPKYYADGEDAYAMKRDLTQMADELRRHLELKEKGRHVVLGAIENKVESKGNSPPSSGEACREEKGLAAEDSGGDSKDLSEVSETTESTDVKDSSEASDSAS

相同性解析による交差性が予測される生物種
Full Name ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC000308
Gene Symbol ARD1A
Gene ID (NCBI) 8260
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP41227
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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