Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-Fro

WB analysis using 16290-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 16290-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16290-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 16290-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 16290-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 16290-1-AP
IP result of anti-ARC/ARG3.1 (IP:16290-1-AP, 4ug; Detection:16290-1-AP 1:300) with mouse brain tissue lysate 4000ug.
IHC staining of mouse brain using 16290-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 16290-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of mouse brain using 16290-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ARC/ARG3.1 antibody (16290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat brain using 16290-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using ARC/ARG3.1 antibody (16290-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

ARC/ARG3.1 Polyclonal antibody

ARC/ARG3.1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 16290-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

ARC, Activity-regulated cytoskeleton-associated protein, Activity-regulated gene 3.1 protein homolog, Arg3.1, hArc

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, mouse brain tissue, rat heart tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF-Fro detected in	rat brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 22 publications below
IHC	See 7 publications below
IF	See 9 publications below
IP	See 1 publications below

Product Information

16290-1-AP targets ARC/ARG3.1 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9445 Product name: Recombinant human ARC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC012321 Sequence: MELDHRTSGGLHAYPGPRGGQVAKPNVILQIGKCRAEMLEHVRRTHRHLLAEVSKQVERELKGLHRSVGKLESNLDGYVPTSDSQRWKKSIKACLCRCQETIANLERWVKREMHVWREVFYRLERWADRLESTGGKYPVGSESARHTVSVGVGGPESYCHEADGYDYTVSPYAITPPPAAGELPGQEPAEAQQYQPWVPGEDGQPSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRKRDLYQTLYVDADEEEIIQYVVGTLQPKLKRFLRHPLPKTLEQLIQRGMEVQDDLEQAAEPAGPHLPVEDEAETLTPAPNSESVASDRTQPE 相同性解析による交差性が予測される生物種
Full Name	activity-regulated cytoskeleton-associated protein
Calculated molecular weight	396 aa, 45 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC012321
Gene Symbol	ARC
Gene ID (NCBI)	23237
RRID	AB_2151832
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7LC44
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration.

Protocols

Product Specific Protocols
IHC protocol for ARC/ARG3.1 antibody 16290-1-AP	Download protocol
IP protocol for ARC/ARG3.1 antibody 16290-1-AP	Download protocol
WB protocol for ARC/ARG3.1 antibody 16290-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation. Authors - Yuqian Shen View Article
mouse	WB	Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Authors - Xiaohui Li View Article
rat	WB,IHC	Aging (Albany NY) EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment. Authors - Zhao-Hui Yao View Article
human	WB	Aging (Albany NY) Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity. Authors - Chenhaoyi Xu View Article
human	WB	Neurobiol Dis Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus. Authors - Daniel Ojeda-Juarez View Article
mouse	WB,IP,IF	Front Mol Neurosci Activity-Dependent Arc Expression and Homeostatic Synaptic Plasticity Are Altered in Neurons from a Mouse Model of Angelman Syndrome. Authors - Elissa D Pastuzyn View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16290-1-AP

WB analysis of SH-SY5Y using 16290-1-AP

WB analysis of mouse brain using 16290-1-AP

WB analysis of rat heart using 16290-1-AP

WB analysis of SH-SY5Y using 16290-1-AP

IP experiment of mouse brain using 16290-1-AP

IHC staining of mouse brain using 16290-1-AP

IHC staining of human brain using 16290-1-AP

IF Staining of mouse brain using 16290-1-AP

IF Staining of rat brain using 16290-1-AP

ARC/ARG3.1 Polyclonal antibody

ARC/ARG3.1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.

Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity.

Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus.

Activity-Dependent Arc Expression and Homeostatic Synaptic Plasticity Are Altered in Neurons from a Mouse Model of Angelman Syndrome.