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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARAP1 Polyclonal antibody (17541-1-AP)

    WB analysis using 17541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17541-1-AP (ARAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARAP1 Polyclonal antibody (17541-1-AP)

    IHC staining of human kidney using 17541-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17541-1-AP (ARAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARAP1 Polyclonal antibody (17541-1-AP)

    IHC staining of human kidney using 17541-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17541-1-AP (ARAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ARAP1 Polyclonal antibody (17541-1-AP)

    IHC staining of mouse heart using 17541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17541-1-AP (ARAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARAP1 Polyclonal antibody

ARAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17541-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1, Centaurin-delta-2, CENTD2, Cnt-d2, KIAA0782

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, L02 cells
Positive IHC detected inhuman kidney tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17541-1-AP targets ARAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11597

Product name: Recombinant human ARAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 329-679 aa of BC056401

Sequence: LYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRMSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

相同性解析による交差性が予測される生物種
Full Name ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Calculated molecular weight162 kDa
Observed molecular weight180 kDa
GenBank accession numberBC056401
Gene Symbol ARAP1
Gene ID (NCBI) 116985
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARAP1 is a member of ARAP family with both Arf GAP and Rho GAP activities, by which ARAP1 makes a direct link between ADP-ribosylation factor (Arf) GTPases and Rho GTPases signaling. ARAP1 is involved in membrane trafficking and receptor recycling. It supports the plasma membrane recycling of the angiotensin II type 1 receptor (AT1) and regulates the cell-specific trafficking of the pro-death receptor TRAIL-R1/DR4, thereby promoting TRAIL-induced apoptosis in certain cell types. (PMID: 14559250, PMID: 18165900, PMID: 38008882)

Protocols

Product Specific Protocols
IHC protocol for ARAP1 antibody 17541-1-APDownload protocol
WB protocol for ARAP1 antibody 17541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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