Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17541-1-AP targets ARAP1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11597 Product name: Recombinant human ARAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 329-679 aa of BC056401 Sequence: LYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRMSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV 相同性解析による交差性が予測される生物種 |
| Full Name | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 |
| Calculated molecular weight | 1210aa,136 kDa; 679aa,78 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | BC056401 |
| Gene Symbol | ARAP1 |
| Gene ID (NCBI) | 116985 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P48 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARAP1 is a member of ARAP family with both Arf GAP and Rho GAP activities, by which ARAP1 makes a direct link between ADP-ribosylation factor (Arf) GTPases and Rho GTPases signaling. ARAP1 is involved in membrane trafficking and receptor recycling. It supports the plasma membrane recycling of the angiotensin II type 1 receptor (AT1) and regulates the cell-specific trafficking of the pro-death receptor TRAIL-R1/DR4, thereby promoting TRAIL-induced apoptosis in certain cell types. (PMID: 14559250, PMID: 18165900, PMID: 38008882)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARAP1 antibody 17541-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |