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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AQR Polyclonal antibody

AQR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24342-1-AP

Synonyms

AQR, aquarius homolog (mouse), fSAP164, IBP160, KIAA0560

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 24342-1-AP

    WB analysis of HEK-293 using 24342-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 24342-1-AP

    WB analysis of Jurkat using 24342-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 24342-1-AP

    WB analysis of HEK-293 using 24342-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 24342-1-AP

    IP experiment of HEK-293 using 24342-1-AP

    IP result of anti-AQR (IP:24342-1-AP, 4ug; Detection:24342-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

  • IHC staining of human brain using 24342-1-AP

    IHC staining of human brain using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 24342-1-AP

    IHC staining of human kidney using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 24342-1-AP

    IHC staining of human kidney using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

  • IHC staining of human brain using 24342-1-AP

    IHC staining of human brain using 24342-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24342-1-AP is AQR Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AQR fusion protein Ag17810 相同性解析による交差性が予測される生物種
Full Name aquarius homolog (mouse)
Calculated molecular weight 1485 aa, 171 kDa
Observed molecular weight 160-171 kDa
GenBank accession numberBC127111
Gene symbol AQR
Gene ID (NCBI) 9716
RRIDAB_2879501
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AQR antibody 24342-1-APDownload protocol
IHC protocol for AQR antibody 24342-1-APDownload protocol
IP protocol for AQR antibody 24342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability.

Authors - Federico Teloni
View Article