Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis using 20334-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 20334-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 20334-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 20334-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 20334-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of rat lung using 20334-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AQP5 antibody (20334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AQP5 Polyclonal antibody

AQP5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 20334-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

aquaporin 5, AQP 5, AQP-5, aquaporin5, Aquaporin-5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (43)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse lung tissue, rat lung tissue
Positive IHC detected in	mouse lung tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	rat lung tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 16 publications below
IHC	See 8 publications below
IF	See 18 publications below

Product Information

20334-1-AP targets AQP5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14103 Product name: Recombinant human AQP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 190-265 aa of BC032946 Sequence: FGPAVVMNRFSPAHWVFWVGPIVGAVLAAILYFYLLFPNSLSLSERVAIIKGTYEPDEDWEEQREERKKTMELTTR 相同性解析による交差性が予測される生物種
Full Name	aquaporin 5
Calculated molecular weight	265 aa, 28 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC032946
Gene Symbol	AQP5
Gene ID (NCBI)	362
RRID	AB_2918066
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P55064
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.

Protocols

Product Specific Protocols
IF protocol for AQP5 antibody 20334-1-AP	Download protocol
IHC protocol for AQP5 antibody 20334-1-AP	Download protocol
WB protocol for AQP5 antibody 20334-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun FGF 13 functions as a regulator of the ERK/aerobic glycolysis axis in the inflammatory state during septic lung injury. Authors - Junjie Zhu View Article
		Redox Biol Norepinephrine attenuates acute lung injury by protecting mitochondria via the β(2)-AR-AKAP1 axis and inhibiting alveolar epithelial pyroptosis. Authors - Yiyuan Yin View Article
		Redox Biol Redox signaling-mediated S-glutathionylation of protein disulfide isomerase A1 initiates intrinsic apoptosis and contributes to accelerated aging Authors - Zhi-Wei Ye View Article
rat	WB	Theranostics Exosomes of human adipose stem cells mitigate irradiation injury to salivary glands by inhibiting epithelial-mesenchymal transition through miR-199a-3p targeting Twist1 and regulating TGFβ1/Smad3 pathway Authors - Xiaotong Guo View Article
mouse	IF	Biomaterials Inhaled pulmonary surfactant biomimetic liposomes for reversing idiopathic pulmonary fibrosis through synergistic therapeutic strategy Authors - Binghua Wang View Article
mouse	IHC	Phytomedicine β-Eudesmol in Rhizoma Atractylodis targets AVPR2 to inhibit the cAMP-AQP2 pathway and promote fluid metabolism Authors - Chang Ke View Article

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Validation Data Gallery

WB analysis using 20334-1-AP

IHC staining of mouse lung using 20334-1-AP

IHC staining of mouse lung using 20334-1-AP

IHC staining of rat kidney using 20334-1-AP

IHC staining of rat kidney using 20334-1-AP

IF Staining of rat lung using 20334-1-AP

AQP5 Polyclonal antibody

AQP5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FGF 13 functions as a regulator of the ERK/aerobic glycolysis axis in the inflammatory state during septic lung injury.

Norepinephrine attenuates acute lung injury by protecting mitochondria via the β(2)-AR-AKAP1 axis and inhibiting alveolar epithelial pyroptosis.

Redox signaling-mediated S-glutathionylation of protein disulfide isomerase A1 initiates intrinsic apoptosis and contributes to accelerated aging

Exosomes of human adipose stem cells mitigate irradiation injury to salivary glands by inhibiting epithelial-mesenchymal transition through miR-199a-3p targeting Twist1 and regulating TGFβ1/Smad3 pathway

Inhaled pulmonary surfactant biomimetic liposomes for reversing idiopathic pulmonary fibrosis through synergistic therapeutic strategy

β-Eudesmol in Rhizoma Atractylodis targets AVPR2 to inhibit the cAMP-AQP2 pathway and promote fluid metabolism

Able^™

Validation Data Gallery

WB analysis using 20334-1-AP

IHC staining of mouse lung using 20334-1-AP

IHC staining of mouse lung using 20334-1-AP

IHC staining of rat kidney using 20334-1-AP

IHC staining of rat kidney using 20334-1-AP

IF Staining of rat lung using 20334-1-AP

AQP5 Polyclonal antibody

AQP5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FGF 13 functions as a regulator of the ERK/aerobic glycolysis axis in the inflammatory state during septic lung injury.

Norepinephrine attenuates acute lung injury by protecting mitochondria via the β(2)-AR-AKAP1 axis and inhibiting alveolar epithelial pyroptosis.

Redox signaling-mediated S-glutathionylation of protein disulfide isomerase A1 initiates intrinsic apoptosis and contributes to accelerated aging

Exosomes of human adipose stem cells mitigate irradiation injury to salivary glands by inhibiting epithelial-mesenchymal transition through miR-199a-3p targeting Twist1 and regulating TGFβ1/Smad3 pathway

Inhaled pulmonary surfactant biomimetic liposomes for reversing idiopathic pulmonary fibrosis through synergistic therapeutic strategy

β-Eudesmol in Rhizoma Atractylodis targets AVPR2 to inhibit the cAMP-AQP2 pathway and promote fluid metabolism

Able™

Able^™