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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AQP4 Monoclonal antibody

AQP4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G7D3

Cat no : 68448-1-Ig

Synonyms

AQP 4, AQP4, aquaporin 4, HMIWC2, MIWC, WCH4

Validation Data Gallery

Applications:
  • WB analysis using 68448-1-Ig

    WB analysis using 68448-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat spinal cord using 68448-1-Ig

    WB analysis of rat spinal cord using 68448-1-Ig

    rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig brain tissue, rat spinal cord tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68448-1-Ig targets AQP4 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AQP4 fusion protein Ag9830 相同性解析による交差性が予測される生物種
Full Name aquaporin 4
Calculated molecular weight 323 aa, 35 kDa
Observed molecular weight35-37 kDa
GenBank accession numberBC022286
Gene symbol AQP4
Gene ID (NCBI) 361
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for AQP4 antibody 68448-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols