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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using APPL2 Polyclonal antibody (14294-1-AP)

    WB analysis of BxPC-3 using 14294-1-AP (same clone as 14294-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using APPL2 Polyclonal antibody (14294-1-AP)

    WB analysis of BxPC-3 using 14294-1-AP (same clone as 14294-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14294-1-AP (APPL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of mouse brain using 14294-1-AP (same clone as 14294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of mouse brain using 14294-1-AP (same clone as 14294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of human malignant melanoma using 14294-1-AP (same clone as 14294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using APPL2 Polyclonal antibody (14294-1-AP)

    IHC staining of human pancreas cancer using 14294-1-AP (same clone as 14294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14294-1-AP (APPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using APPL2 Polyclonal antibody (14294-1-AP)

    IF Staining of BxPC-3 using 14294-1-AP (same clone as 14294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using APPL2 antibody (14294-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14294-1-PBS in a different storage buffer formulation.

APPL2 Polyclonal antibody, PBS Only

APPL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14294-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Dip13-beta, DIP13B, Dip13 beta, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14294-1-PBS targets APPL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5626

Product name: Recombinant human APPL2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 314-664 aa of BC033731

Sequence: RGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKVTASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Calculated molecular weight 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC033731
Gene Symbol APPL2
Gene ID (NCBI) 55198
RRIDAB_2878041
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEU8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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