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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APPL1 Monoclonal antibody (68195-1-Ig)

    WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APPL1 Monoclonal antibody (68195-1-Ig)

    WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APPL1 Monoclonal antibody (68195-1-Ig)

    WB analysis using 68195-1-Ig (same clone as 68195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using APPL1 Monoclonal antibody (68195-1-Ig)

    WB analysis of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using APPL1 Monoclonal antibody (68195-1-Ig)

    WB analysis of pig heart using 68195-1-Ig (same clone as 68195-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using APPL1 Monoclonal antibody (68195-1-Ig)

    WB analysis of HEK-293 using 68195-1-Ig (same clone as 68195-1-PBS)

    WB result of APPL1 antibody (68195-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using APPL1 Monoclonal antibody (68195-1-Ig)

    IHC staining of human colon cancer using 68195-1-Ig (same clone as 68195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68195-1-Ig (APPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APPL1 Monoclonal antibody (68195-1-Ig)

    IF Staining of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (68195-1-Ig, Clone: 1B7B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using APPL1 Monoclonal antibody (68195-1-Ig)

    FC experiment of HepG2 using 68195-1-Ig (same clone as 68195-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (68195-1-Ig, Clone:1B7B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68195-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

APPL1 Monoclonal antibody, PBS Only

APPL1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68195-1-PBS
Clone No.1B7B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

1B7B11, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, APPL, DCC-interacting protein 13-alpha, Dip13 alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68195-1-PBS targets APPL1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3334

Product name: Recombinant human APPL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 405-709 aa of BC028599

Sequence: QQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Calculated molecular weight 709 aa, 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC028599
Gene Symbol APPL1
Gene ID (NCBI) 26060
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UKG1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

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