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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APPBP2 Polyclonal antibody (12409-1-AP)

    WB analysis using 12409-1-AP (same clone as 12409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APPBP2 Polyclonal antibody (12409-1-AP)

    WB analysis using 12409-1-AP (same clone as 12409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4. This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4. This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4. This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using APPBP2 Polyclonal antibody (12409-1-AP)

    IHC staining of human breast cancer using 12409-1-AP (same clone as 12409-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4. This data was developed using the same antibody clone with 12409-1-PBS in a different storage buffer formulation.

APPBP2 Polyclonal antibody, PBS Only

APPBP2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12409-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12409-1-PBS targets APPBP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3092

Product name: Recombinant human APPBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 288-586 aa of BC018121

Sequence: SDTLLDYGFYLLNVDNICQSVAIYQAALDIRQSVFGGKNIHVATAHEDLAYSSYVHQYSSGKFDNALFHAERAIGIITHILPEDHLLLASSKRVKALILEEIAIDCHNKETEQRLLQEAHDLHLSSLQLAKKAFGEFNVQTAKHYGNLGRLYQSMRKFKEAEEMHIKAIQIKEQLLGQEDYEVALSVGHLASLYNYDMNQYENAEKLYLRSIAIGKKLFGEGYSGLEYDYRGLIKLYNSIGNYEKVFEYHNVLSNWNRLRDRQYSVTDALEDVSTSPQSTEEVVQSFLISQNVEGPSC

相同性解析による交差性が予測される生物種
Full Name amyloid beta precursor protein (cytoplasmic tail) binding protein 2
Calculated molecular weight 585 aa, 67 kDa
Observed molecular weight67 kDa
GenBank accession numberBC018121
Gene Symbol APPBP2
Gene ID (NCBI) 10513
RRIDAB_2918029
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92624
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).

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