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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOOL Polyclonal antibody (28514-1-AP)

    WB analysis using 28514-1-AP (same clone as 28514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28514-1-AP (APOOL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human kidney using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using APOOL Polyclonal antibody (28514-1-AP)

    IHC staining of human placenta using 28514-1-AP (same clone as 28514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28514-1-AP (APOOL antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOOL Polyclonal antibody (28514-1-AP)

    IF Staining of HeLa using 28514-1-AP (same clone as 28514-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using APOOL antibody (28514-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28514-1-PBS in a different storage buffer formulation.

APOOL Polyclonal antibody, PBS Only

APOOL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28514-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28514-1-PBS targets APOOL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29392

Product name: Recombinant human APOOL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 127-268 aa of BC107096

Sequence: VSARKGSKFKKITYPLGLATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAVKSLWTKSSKEESLPKPKEKTKLGSSSEIEVPAKTTHVLKHSVPLPTELSSEAKTKSESTSGATQFMPDPKLMDHGQSHPEDIDMYSTRS

相同性解析による交差性が予測される生物種
Full Name apolipoprotein O-like
Calculated molecular weight 268 aa, 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC107096
Gene Symbol APOOL
Gene ID (NCBI) 139322
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXV4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

APOOL, the component of the MICOS complex, is a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:23704930), (PubMed:25764979). Its molecular weight is about 30 kDa.

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