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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOL4 Polyclonal antibody (10564-1-AP)

    WB analysis using 10564-1-AP (same clone as 10564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10564-1-AP (APOL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using APOL4 Polyclonal antibody (10564-1-AP)

    IP experiment of HepG2 using 10564-1-AP (same clone as 10564-1-PBS)

    IP result of anti-APOL4 (IP:10564-1-AP, 4ug; Detection:10564-1-AP 1:2000) with HepG2 cells lysate 4000ug. This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver cancer using 10564-1-AP (same clone as 10564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver cancer using 10564-1-AP (same clone as 10564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver using 10564-1-AP (same clone as 10564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using APOL4 Polyclonal antibody (10564-1-AP)

    IHC staining of human liver using 10564-1-AP (same clone as 10564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10564-1-AP (APOL4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOL4 Polyclonal antibody (10564-1-AP)

    IF Staining of HepG2 using 10564-1-AP (same clone as 10564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL4 antibody (10564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOL4 Polyclonal antibody (10564-1-AP)

    IF Staining of HepG2 using 10564-1-AP (same clone as 10564-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10564-1-AP (APOL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10564-1-PBS in a different storage buffer formulation.

APOL4 Polyclonal antibody, PBS Only

APOL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10564-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10564-1-PBS targets APOL4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0849

Product name: Recombinant human APOL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-107 aa of BC006276

Sequence: MGSWVQLITSVGTSGLFLGVRVREEGAGMRCSKTIQAGQWLDSSKGPLGPSPPPVPTAGYSSSFCVHYVNLLPGVLVLSVTSQYPHLSMALCQLAAHDWPRLSCVCV

相同性解析による交差性が予測される生物種
Full Name apolipoprotein L, 4
Calculated molecular weight 39 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC006276
Gene Symbol APOL4
Gene ID (NCBI) 80832
RRIDAB_2242901
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPW4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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