Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, BxPC-3 cells, HCT 116 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28591-1-AP targets APOL3 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29687 Product name: Recombinant human APOL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 246-380 aa of NM_145640 Sequence: SSAEAEASRLTATSIDRLKVFKEVMRDITPNLLSLLNNYYEATQTIGSEIRAIRQARARARLPVTTWRISAGSGGQAERTIAGTTRAVSRGARILSATTSGIFLALDVVNLVYESKHLHEGAKSASAEELRRQAQ 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein L, 3 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44 kDa |
| GenBank accession number | NM_145640 |
| Gene Symbol | APOL3 |
| Gene ID (NCBI) | 80833 |
| RRID | AB_3669663 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Apolipoprotein L3(APOL3) is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. APOL3 is localized in the cytoplasm and plays a central role in cholesterol transport, affecting the movement of lipids, including cholesterol, and allowing the binding of lipids to organelles.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APOL3 antibody 28591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |