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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Apolipoprotein CIII Polyclonal antibody (22262-1-AP)

    WB analysis of human plasma using 22262-1-AP (same clone as 22262-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 22262-1-AP (APOC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Apolipoprotein CIII Polyclonal antibody (22262-1-AP)

    IHC staining of human liver using 22262-1-AP (same clone as 22262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22262-1-AP (APOC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Apolipoprotein CIII Polyclonal antibody (22262-1-AP)

    IHC staining of human liver using 22262-1-AP (same clone as 22262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22262-1-AP (APOC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22262-1-PBS in a different storage buffer formulation.

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Apolipoprotein CIII Polyclonal antibody, PBS Only

Apolipoprotein CIII Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22262-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

APOC3, Apo CIII, ApoC III, APOCIII, Apo-CIII

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22262-1-PBS targets Apolipoprotein CIII in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name apolipoprotein C-III
Calculated molecular weight 11 kDa
Observed molecular weight11 kDa
GenBank accession numberNM_000040
Gene Symbol Apolipoprotein CIII
Gene ID (NCBI) 345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02656
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Apolipoprotein CIII (apoCIII) is the most abundant C-apolipoprotein in human and is found on very low density lipoproteins, chylomicrons, and high density lipoproteins (HDL) (PMID: 18408013). ApoCIII inhibits lipoprotein lipase as well as binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors (PMID: 26228667). ApoC-III has 3 actions that can impair plasma lipoprotein metabolism and lead to hypertriglyceridemia (PMID: 24941081).