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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using APOBEC4 Polyclonal antibody (17166-1-AP)

    WB analysis of HeLa using 17166-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17166-1-AP (APOBEC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using APOBEC4 Polyclonal antibody (17166-1-AP)

    WB analysis of HeLa using 17166-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17166-1-AP (APOBEC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human testis using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human testis using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human skin using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human skin using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human brain using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human brain using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human liver using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human liver using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human spleen using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human spleen using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human lung using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human lung using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human ovary using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human ovary using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human heart using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human heart using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human placenta using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using APOBEC4 Polyclonal antibody (17166-1-AP)

    IHC staining of human placenta using 17166-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APOBEC4 Polyclonal antibody (17166-1-AP)

    IF Staining of HeLa using 17166-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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APOBEC4 Polyclonal antibody

APOBEC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17166-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Putative C->U-editing enzyme APOBEC-4, EC:3.5.4.-, C1orf169, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17166-1-AP targets APOBEC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOBEC4 fusion protein Ag10633 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative)
Calculated molecular weight 367 aa, 42 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC021711
Gene Symbol APOBEC4
Gene ID (NCBI) 403314
RRIDAB_2057526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WW27
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for APOBEC4 antibody 17166-1-APDownload protocol
IHC protocol for APOBEC4 antibody 17166-1-APDownload protocol
IF protocol for APOBEC4 antibody 17166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols