Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 17166-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17166-1-AP (APOBEC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17166-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17166-1-AP (APOBEC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse testis using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17166-1-AP (APOBEC4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17166-1-AP (APOBEC4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 17166-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 17166-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17166-1-AP (APOBEC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

APOBEC4 Polyclonal antibody

APOBEC4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17166-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 4, C1orf169, EC:3.5.4.-, Putative C->U-editing enzyme APOBEC-4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human testis tissue, human lung tissue, human brain tissue, human heart tissue, human skin tissue, human ovary tissue, mouse testis tissue, human spleen tissue, human liver tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

17166-1-AP targets APOBEC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10633 Product name: Recombinant human APOBEC4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-367 aa of BC021711 Sequence: MEPIYEEYLANHGTIVKPYYWLSFSLDCSNCPYHIRTGEEARVSLTEFCQIFGFPYGTTFPQTKHLTFYELKTSSGSLVQKGHASSCTGNYIHPESMLFEMNGYLDSAIYNNDSIRHIILYSNNSPCNEANHCCISKMYNFLITYPGITLSIYFSQLYHTEMDFPASAWNREALRSLASLWPRVVLSPISGGIWHSVLHSFISGVSGSHVFQPILTGRALADRHNAYEINAITGVKPYFTDVLLQTKRNPNTKAQEALESYPLNNAFPGQFFQMPSGQLQPNLPPDLRAPVVFVLVPLRDLPPMHMGQNPNKPRNIVRHLNMPQMSFQETKDLGRLPTGRSVEIVEITEQFASSKEADEKKKKKGKK 相同性解析による交差性が予測される生物種
Full Name	apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative)
Calculated molecular weight	367 aa, 42 kDa
Observed molecular weight	45-48 kDa
GenBank accession number	BC021711
Gene Symbol	APOBEC4
Gene ID (NCBI)	403314
RRID	AB_2057526
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WW27
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for APOBEC4 antibody 17166-1-AP	Download protocol
IHC protocol for APOBEC4 antibody 17166-1-AP	Download protocol
WB protocol for APOBEC4 antibody 17166-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Adv Res UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis. Authors - Peizhuo Pang View Article

Validation Data Gallery

WB analysis of HeLa using 17166-1-AP

WB analysis of HeLa using 17166-1-AP

IHC staining of human testis using 17166-1-AP

IHC staining of human testis using 17166-1-AP

IHC staining of mouse testis using 17166-1-AP

IHC staining of human testis using 17166-1-AP

IHC staining of human heart using 17166-1-AP

IHC staining of human heart using 17166-1-AP

IHC staining of human placenta using 17166-1-AP

IHC staining of human placenta using 17166-1-AP

IHC staining of human skin using 17166-1-AP

IHC staining of human skin using 17166-1-AP

IHC staining of human brain using 17166-1-AP

IHC staining of human brain using 17166-1-AP

IHC staining of human liver using 17166-1-AP

IHC staining of human liver using 17166-1-AP

IHC staining of human spleen using 17166-1-AP

IHC staining of human spleen using 17166-1-AP

IHC staining of human lung using 17166-1-AP

IHC staining of human lung using 17166-1-AP

IHC staining of human ovary using 17166-1-AP

IHC staining of human ovary using 17166-1-AP

IF Staining of HeLa using 17166-1-AP

APOBEC4 Polyclonal antibody

APOBEC4 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis.

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