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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOBEC2 Polyclonal antibody (20121-1-AP)

    WB analysis using 20121-1-AP (same clone as 20121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20121-1-AP (APOBEC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IP experiment of mouse skeletal muscle using 20121-1-AP (same clone as 20121-1-PBS)

    IP result of anti-APOBEC2 (IP:20121-1-AP, 4ug; Detection:20121-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of human heart using 20121-1-AP (same clone as 20121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using APOBEC2 Polyclonal antibody (20121-1-AP)

    IHC staining of human heart using 20121-1-AP (same clone as 20121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

APOBEC2 Polyclonal antibody, PBS Only

APOBEC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20121-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20121-1-PBS targets APOBEC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13699

Product name: Recombinant human APOBEC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC047767

Sequence: MAQKEEAAVATEAASQNGEDLENLDDPEKLKELIELPPFEIVTGERLPANFFKFQFRNVEYSSGRNKTFLCYVVEAQGKGGQVQASRGYLEDEHAAAHAEEAFFNTILPAFDPALRYNVTWYVSSSPCAACADRIIKTLSKTKNLRLLILVGRLFMWEEPEIQAALKKLKEAGCKLRIMKPQDFEYVWQNFVEQEEGESKAFQPWEDIQENFLYYEEKLADILK

相同性解析による交差性が予測される生物種
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
Calculated molecular weight 26 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC047767
Gene Symbol APOBEC2
Gene ID (NCBI) 10930
RRIDAB_10697687
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y235
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

APOBEC2, also named as ARCD1 and ARP1, is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide (APOBEC) cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. APOBEC family members have diverse roles by their ability to edit DNA and/or RNA. Apobec2 is one of the oldest members of the APOBEC family, along with the lymphoid-specific activation-induced deaminase. Unlike other APOBEC family members, the enzymatic activity and substrate of Apobec2 have not been fully demonstrated, and its biologic functions remain unknown. It can be detected as 25-30 kDa.

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