Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32857-1-AP targets APOBEC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26715 Product name: Recombinant human APOBEC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of NM_001304566 Sequence: MTSEKGPSTGDPTLRRRIEPWEFDVFYDPRELRKEACLLYEIKWGMSRKIWRSSGKNTTNHVEVNFIKKFTSERDFHPSMSCSITWFLSWSPCWECSQAIREFLSRHPGVTLVIYVARLFWHMDQQNRQGLRDLV 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | NM_001304566 |
| Gene Symbol | APOBEC1 |
| Gene ID (NCBI) | 339 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P41238 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Apolipoprotein B mRNA-editing enzyme catalytic subunit 1 (APOBEC1) is a member of the cytidine deaminase enzyme family. It forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP) (PMID: 30844405). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs (PMID: 24916387; 11727199). APOBEC1 plays a crucial role in RNA editing, which is essential for the proper function of these proteins. Additionally, APOBEC1 has been implicated in various cellular processes, including the regulation of gene expression and the maintenance of genomic stability (PMID: 33094286).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APOBEC1 antibody 32857-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |