Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25328-1-AP targets APOB48R in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18204 Product name: Recombinant human APOB48R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 851-1088 aa of BC119786 Sequence: SQTARAEGMGAMVEAGGLLEKWTLLEEEAVGWQEREQREDSEGRCGDYHPEGEAPRLLDAEGLMVTGGRRAEAKETEPESLEHVRGQEEQPTHQAPAEAAPESVGEAETAEAMGSARGGAANSWSEAPLPGSLLDVSVPRSRVHLSRSSSQRRSRPSFRRTPAWEQQEEPPAPNPPEEELSAPEQRPLQLEEPLEPSPLRHDGTPVPARRRPLGHGFGLAHPGMMQELQARLGRPKPQ 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein B48 receptor |
| Calculated molecular weight | 1088 aa, 116 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC119786 |
| Gene Symbol | APOB48R |
| Gene ID (NCBI) | 55911 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q0VD83 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Apolipoprotein B48 receptor (APOB48R) is a macrophage receptor that binds to apoB of dietary triglyceride-rich lipoproteins or to a similar apoB-like domain in hypertriglyceridemic very low-density lipoprotein (HTG-VLDL). This receptor is crucial for the uptake of chylomicrons, HTG-VLDL, and trypsinized VLDL lacking apoE in macrophages. APOB48R may contribute necessary lipids, lipid-soluble vitamins, and other nutrients to reticuloendothelial cells. Elevated plasma triglyceride levels may cause APOB48R to be a factor in foam cell formation, endothelial dysfunction, and atherothrombogenesis (PMID: 10852956; 9633939; 15591219).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APOB48R antibody 25328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |