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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb™ APOB Multi-Recombinant antibody (RMX00011)

    WB analysis using RMX00011

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00011 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Multi-rAb™ APOB Multi-Recombinant antibody (RMX00011)

    IHC staining of mouse liver using RMX00011

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00011 (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Multi-rAb™ APOB Multi-Recombinant antibody (RMX00011)

    IF Staining of mouse liver using RMX00011

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (RMX00011) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Multi-rAb™ APOB Multi-Recombinant antibody

APOB Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. RMX00011

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Apolipoprotein B, Apolipoprotein B-100, ApoB48, ApoB100, Apo B-100

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00011 targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name apolipoprotein B (including Ag(x) antigen)
Calculated molecular weight 516 kDa
Observed molecular weight150-250 kDa, 400-520 kDa
GenBank accession numberNM_000384
Gene Symbol APOB
Gene ID (NCBI) 338
Conjugate Unconjugated
Form Liquid
Purification MethodN/A
UNIPROT IDP04114
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

Protocols

Product Specific Protocols
WB protocol for APOB antibody RMX00011Download protocol
IHC protocol for APOB antibody RMX00011Download protocol
IF protocol for APOB antibody RMX00011Download protocol
Standard Protocols
Click here to view our Standard Protocols