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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    WB analysis of HepG2 using 83504-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    IHC staining of human stomach cancer using 83504-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83504-1-RR (Apolipoprotein AI antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    IF Staining of HepG2 using 83504-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein AI antibody (83504-1-RR, Clone: 240269F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using Apolipoprotein AI Recombinant antibody (83504-1-RR)

    FC experiment of HepG2 using 83504-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Apolipoprotein AI Recombinant antibody (83504-1-RR, Clone:240269F1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83504-1-RR

    Affinity and Kinetic Characterization of 83504-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83504-1-RR against Human Apolipoprotein AI were performed. The affinity constant is 4.05 nM.

Apolipoprotein AI Recombinant monoclonal antibody

Apolipoprotein AI Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83504-1-RR
Clone No.240269F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

APOA1, apolipoprotein A I, 240269F1, APOA 1, ApoA I

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83504-1-RR targets Apolipoprotein AI in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0757

Product name: Recombinant Human APOA1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-267 aa of BC005380

Sequence: DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ

相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene Symbol APOA1
Gene ID (NCBI) 335
RRIDAB_3671129
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP02647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis.

Protocols

Product Specific Protocols
FC protocol for Apolipoprotein AI antibody 83504-1-RRDownload protocol
IHC protocol for Apolipoprotein AI antibody 83504-1-RRDownload protocol
WB protocol for Apolipoprotein AI antibody 83504-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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