Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IHC | See 1 publications below |
Product Information
66206-1-Ig targets Apolipoprotein AI in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21920 Product name: Recombinant human APOA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-267 aa of BC005380 Sequence: RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ 相同性解析による交差性が予測される生物種 |
| Full Name | apolipoprotein A-I |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 26-28 kDa |
| GenBank accession number | BC005380 |
| Gene Symbol | APOA1 |
| Gene ID (NCBI) | 335 |
| RRID | AB_2881597 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02647 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Apolipoprotein AI antibody 66206-1-Ig | Download protocol |
| IHC protocol for Apolipoprotein AI antibody 66206-1-Ig | Download protocol |
| WB protocol for Apolipoprotein AI antibody 66206-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles | ||
EMBO J Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes | ||
Mol Cell Proteomics Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice. | ||
Peptides Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor. | ||
Mol Biol Rep Differential proteomic profiles of exosomes in pediatric and adult adamantinomatous craniopharyngioma cyst fluid |
