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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APCDD1 Polyclonal antibody

APCDD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17024-1-AP

Synonyms

APCDD1, B7323, DRAPC1, FP7019, Protein APCDD1

Validation Data Gallery

Applications:
  • WB analysis using 17024-1-AP

    WB analysis using 17024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17024-1-AP (APCDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 17024-1-AP

    IHC staining of human heart using 17024-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17024-1-AP (APCDD1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 17024-1-AP

    IHC staining of human heart using 17024-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17024-1-AP (APCDD1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inCOLO 320 cells, Raji cells, mouse brain tissue, mouse heart tissue, mouse skin tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17024-1-AP targets APCDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APCDD1 fusion protein Ag10303 相同性解析による交差性が予測される生物種
Full Name adenomatosis polyposis coli down-regulated 1
Calculated molecular weight 514 aa, 59 kDa
Observed molecular weight68 kDa
GenBank accession numberBC053324
Gene symbol APCDD1
Gene ID (NCBI) 147495
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562).

Protocols

Product Specific Protocols
WB protocol for APCDD1 antibody 17024-1-APDownload protocol
IHC protocol for APCDD1 antibody 17024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols