Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 21748-1-AP (same clone as 21748-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
WB analysis using 21748-1-AP (same clone as 21748-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 21748-1-AP (same clone as 21748-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
WB analysis of Transfected HEK-293 using 21748-1-AP (same clone as 21748-1-PBS)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (ANAPC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
WB analysis of HT-1080 using 21748-1-AP (same clone as 21748-1-PBS)
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21748-1-AP (same clone as 21748-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 21748-1-AP (same clone as 21748-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21748-1-AP (APC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 21748-1-AP (same clone as 21748-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 21748-1-AP (same clone as 21748-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21748-1-AP (APC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 21748-1-AP (same clone as 21748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 21748-1-AP (APC1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 21748-1-AP (same clone as 21748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 21748-1-AP (APC1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 21748-1-AP (same clone as 21748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach using 21748-1-AP (APC1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 21748-1-AP (same clone as 21748-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach using 21748-1-AP (APC1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21748-1-PBS in a different storage buffer formulation.

APC1 Polyclonal antibody, PBS Only

APC1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21748-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ANAPC1, Anaphase-promoting complex subunit 1, Apc 1, Mitotic checkpoint regulator, Testis-specific gene 24 protein

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21748-1-PBS targets APC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13399 Product name: Recombinant human ANAPC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1596-1944 aa of BC104902 Sequence: STDNRYHLQALRHLYVLAAEPRLLVPVDVDTNTPCYALLEVTYKGTQWYEQTKEELMAPTLLPELHLLKQIKVKGPRYWELLIDLSKGTQHLKSILSKDGVLYVKLRAGQLSYKEDPMGWQSLLAQTVANRNSEARAFKPETISAFTSDPALLSFAEYFCKPTVNMGQKQEILDLFSSVLYECVTQETPEMLPAYIAMDQAIRRLGRREMSETSELWQIKLVLEFFSSRSHQERLQNHPKRGLFMNSEFLPVVKCTIDNTLDQWLQVGGDMCVHAYLSGQPLEESQLSMLACFLVYHSVPAPQHLPPIGLEGSTSFAELLFKFKQLKMPVRALLRLAPLLLGNPQPMVM 相同性解析による交差性が予測される生物種
Full Name	anaphase promoting complex subunit 1
Calculated molecular weight	1944 aa, 217 kDa
Observed molecular weight	200-210 kDa
GenBank accession number	BC104902
Gene Symbol	APC1
Gene ID (NCBI)	64682
RRID	AB_10733241
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1A4
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 21748-1-AP (same clone as 21748-1-PBS)

WB analysis using 21748-1-AP (same clone as 21748-1-PBS)

WB analysis of K-562 using 21748-1-AP (same clone as 21748-1-PBS)

WB analysis of Transfected HEK-293 using 21748-1-AP (same clone as 21748-1-PBS)

WB analysis of HT-1080 using 21748-1-AP (same clone as 21748-1-PBS)

WB analysis of HEK-293 using 21748-1-AP (same clone as 21748-1-PBS)

WB analysis of mouse brain using 21748-1-AP (same clone as 21748-1-PBS)

IHC staining of mouse testis using 21748-1-AP (same clone as 21748-1-PBS)

IHC staining of mouse testis using 21748-1-AP (same clone as 21748-1-PBS)

IHC staining of human cervical cancer using 21748-1-AP (same clone as 21748-1-PBS)