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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using APBB2 Polyclonal antibody (13177-1-AP)

    WB analysis of human brain using 13177-1-AP (same clone as 13177-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13177-1-AP (APBB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using APBB2 Polyclonal antibody (13177-1-AP)

    IHC staining of human placenta using 13177-1-AP (same clone as 13177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using APBB2 Polyclonal antibody (13177-1-AP)

    IHC staining of human stomach cancer using 13177-1-AP (same clone as 13177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using APBB2 Polyclonal antibody (13177-1-AP)

    IHC staining of mouse stomach using 13177-1-AP (same clone as 13177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using APBB2 Polyclonal antibody (13177-1-AP)

    IHC staining of rat kidney using 13177-1-AP (same clone as 13177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using APBB2 Polyclonal antibody (13177-1-AP)

    IHC staining of mouse pancreas using 13177-1-AP (same clone as 13177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using APBB2 Polyclonal antibody (13177-1-AP)

    IHC staining of mouse kidney using 13177-1-AP (same clone as 13177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13177-1-AP (APBB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APBB2 Polyclonal antibody (13177-1-AP)

    IF Staining of HeLa using 13177-1-AP (same clone as 13177-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13177-1-AP (APBB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13177-1-PBS in a different storage buffer formulation.

APBB2 Polyclonal antibody, PBS Only

APBB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13177-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FE65L, FE65L1, A4, Amyloid-beta, Protein Fe65 like 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13177-1-PBS targets APBB2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3844

Product name: Recombinant human APBB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 386-736 aa of BC027946

Sequence: SCSINSDPEAKCFAVRSLGWVEMAEEDLAPGKSSVAVNNCIRQLSYCKNDIRDTVGIWGEGKDMYLILENDMLSLVDPMDRSVLHSQPIVSIRVWGVGRDNGRDFAYVARDKDTRILKCHVFRCDTPAKAIATSLHEICSKIMAERKNAKALACSSLQERANVNLDVPLQDFPTPKTELVQKFHVQYLGMLPVDKPVGMDILNSAIENLMTSSNKEDWLSVNMNVADATVTVISEKNEEEVLVECRVRFLSFMGVGKDVHTFAFIMDTGNQRFECHVFWCEPNAGNVSEAVQAACMLRYQKCLVARPPSQKVRPPPPPADSVTRRVTTNVKRGVLSLIDTLKQKRPVTEMP

相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family B, member 2
Calculated molecular weight 83 kDa
Observed molecular weight 83-95 kDa
GenBank accession numberBC027946
Gene Symbol APBB2
Gene ID (NCBI) 323
RRIDAB_10638434
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92870
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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