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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APBA3 Polyclonal antibody (20812-1-AP)

    WB analysis using 20812-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20812-1-AP (APBA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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APBA3 Polyclonal antibody

APBA3 Polyclonal Antibody for WB, ELISA
Cat No. 20812-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MINT3, APBA 3, X11L2, Amyloid-beta A4 precursor protein-binding family A member 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HT-1080 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20812-1-AP targets APBA3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APBA3 fusion protein Ag14771 相同性解析による交差性が予測される生物種
Full Name amyloid beta (A4) precursor protein-binding, family A, member 3
Calculated molecular weight 575 aa, 61 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC008338
Gene Symbol APBA3
Gene ID (NCBI) 9546
RRIDAB_3669349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO96018
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The APBA (MINT) family is a family of adaptor proteins composed of APBA1 (MINT1), APBA2 (MINT2), and APBA3 (MINT3). These proteins are involved in neuronal protein transport and synaptic function. APBA3 plays an essential role in mediating the transport of APP from the TGN to the plasma membrane (PMID: 24410826).

Protocols

Product Specific Protocols
WB protocol for APBA3 antibody 20812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols