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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using AP4S1 Polyclonal antibody (27202-1-AP)

    IHC staining of mouse brain using 27202-1-AP (same clone as 27202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AP4S1 Polyclonal antibody (27202-1-AP)

    IHC staining of mouse brain using 27202-1-AP (same clone as 27202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using AP4S1 Polyclonal antibody (27202-1-AP)

    IHC staining of human brain using 27202-1-AP (same clone as 27202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using AP4S1 Polyclonal antibody (27202-1-AP)

    IHC staining of human brain using 27202-1-AP (same clone as 27202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27202-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AP4S1 Polyclonal antibody (27202-1-AP)

    IF Staining of mouse brain using 27202-1-AP (same clone as 27202-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27202-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AP4S1 Polyclonal antibody (27202-1-AP)

    IF Staining of mouse brain using 27202-1-AP (same clone as 27202-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27202-1-PBS in a different storage buffer formulation.

AP4S1 Polyclonal antibody, PBS Only

AP4S1 Polyclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 27202-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27202-1-PBS targets AP4S1 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25661

Product name: Recombinant human AP4S1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 5-98 aa of BC001259

Sequence: FLMVNKQGQTRLSKYYEHVDINKRTLLETEVIKSCLSRSNEQCSFIEYKDFKLIYRQYAALFIVVGVNDTENEMAIYEFIHNFVEVLDEYFSRV

相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 4, sigma 1 subunit
Calculated molecular weight 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC001259
Gene Symbol AP4S1
Gene ID (NCBI) 11154
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y587
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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