Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32945-1-AP targets AP3S1 in WB, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37523 Product name: Recombinant human AP3S1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC000804 Sequence: MIKAILIFNNHGKPRLSKFYQPYSEDTQQQIIRETFHLVSKRDENVCNFLEGGLLIGGSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVHNILAEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPARAVSAVKNMNLPEIPRNINIGDISIKVPNLPSFK 相同性解析による交差性が予測される生物種 |
| Full Name | adaptor-related protein complex 3, sigma 1 subunit |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC000804 |
| Gene Symbol | AP3S1 |
| Gene ID (NCBI) | 1176 |
| RRID | AB_3742800 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q92572 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AP-3 complex subunit sigma-1 (AP3S1) is a component of the heterotetrameric adaptor protein complex-3 (AP-3), which plays a central role in intracellular protein trafficking (PMID: 34565296). AP3S1 is also a potential pan-cancer oncogene and plays an essential role in tumorigenesis and cancer immunity. Elevated expression of AP3S1 indicates an immunosuppressive microenvironment and can be used as a potential prognostic biomarker and a target for immunotherapy (PMID: 35874816; 38609993).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AP3S1 antibody 32945-1-AP | Download protocol |
| WB protocol for AP3S1 antibody 32945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |