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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AP3D1 Polyclonal antibody (16454-1-AP)

    WB analysis of HepG2 using 16454-1-AP (same clone as 16454-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AP3D1 Polyclonal antibody (16454-1-AP)

    WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using AP3D1 Polyclonal antibody (16454-1-AP)

    WB analysis of NIH/3T3 using 16454-1-AP (same clone as 16454-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AP3D1 Polyclonal antibody (16454-1-AP)

    WB analysis using 16454-1-AP (same clone as 16454-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16454-1-AP (AP3D1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AP3D1 Polyclonal antibody (16454-1-AP)

    IF Staining of MCF-7 using 16454-1-AP (same clone as 16454-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AP3D1 antibody (16454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16454-1-PBS in a different storage buffer formulation.

AP3D1 Polyclonal antibody, PBS Only

AP3D1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16454-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16454-1-PBS targets AP3D1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9390

Product name: Recombinant human AP3D1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC010065

Sequence: MALKMVKGSIDRMFDKNLQDLVRGIRNHKEDEAKYISQCIDEIKQELKQDNIAVKANAVCKLTYLQMLGYDISWAAFNIIEVMSASKFTFKRIGYLAASQSFHEGTDVIMLTTNQIRKDLSSPSQYDTGVALTGLSCFVTPDLARDLANDIMTLMSHTKPYIRKKAVLIMYKVFLKYPESLRPAFPRLKEKLEDPDPGVQSAAVNVICELARRNPKNYLSLAPLFFKLMTSSTNNWVLIKIIKLFGALTPLGPRLGKKLIEPLTNLIHSTSAMSLLYECVNTVIAVLISLSSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQSHKDLILQCLDD

相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 3, delta 1 subunit
Calculated molecular weight 1153 aa, 130 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC010065
Gene Symbol AP3D1
Gene ID (NCBI) 8943
RRIDAB_10596951
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14617
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

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