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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    WB analysis of rat brain using 18331-1-AP (same clone as 18331-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 18331-1-AP (AP3B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18331-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    WB analysis of mouse brain using 18331-1-AP (same clone as 18331-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18331-1-AP (AP3B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18331-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AP3B2 Polyclonal antibody (18331-1-AP)

    IP experiment of mouse brain using 18331-1-AP (same clone as 18331-1-PBS)

    IP result of anti-AP3B2 (IP:18331-1-AP, 4ug; Detection:18331-1-AP 1:500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 18331-1-PBS in a different storage buffer formulation.

AP3B2 Polyclonal antibody, PBS Only

AP3B2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 18331-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18331-1-PBS targets AP3B2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12861

Product name: Recombinant human AP3B2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 650-1082 aa of BC093739

Sequence: DPSVRNVEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSDPESESESDSKSSSESGSGESSSESDNEDQDEDEEKGRGSESEQSEEDGKRKTKKKVPERKGEASSSDEGSDSSSSSSESEMTSESEEEQLEPASWSRKTPPSSKSAPATKEISLLDLEDFTPPSVQPVSPPAIVSTSLAADLEGLTLTDSTLVPSLLSPVSGVGRQELLHRVAGEGLAVDYTFSRQPFSGDPHMVSVHIHFSNSSDTPIKGLHVGTPKLPAGISIQEFPEIESLAPGESATAVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSENEFKKEQGKLMGMNEITEKLMLPDTCRSDHIVVQKVTATANLGRVPCGTSDEYRFAGRTLTGGSLVLLTLDARPAGAAQLTVNSEKMVIGTMLVKDVIQALTQ

相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 3, beta 2 subunit
Calculated molecular weight 1082 aa, 119 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberBC093739
Gene Symbol AP3B2
Gene ID (NCBI) 8120
RRIDAB_2878531
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13367
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3B2 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

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