• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AP2B1 Monoclonal antibody

AP2B1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3B6

Cat no : 68349-1-Ig

Synonyms

ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1, DKFZp781K0743

Validation Data Gallery

Applications:
  • WB analysis using 68349-1-Ig

    WB analysis using 68349-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68349-1-Ig

    WB analysis of HeLa using 68349-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68349-1-Ig

    WB analysis using 68349-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68349-1-Ig (AP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68349-1-Ig

    IF Staining of HeLa using 68349-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AP2B1 antibody (68349-1-Ig, Clone: 1A3B6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig cerebellum tissue, HeLa cells, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

68349-1-Ig targets AP2B1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AP2B1 fusion protein Ag8264 相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 2, beta 1 subunit
Calculated molecular weight 105 kDa
Observed molecular weight100-115 kDa
GenBank accession numberBC006201
Gene symbol AP2B1
Gene ID (NCBI) 163
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.

Protocols

Product Specific Protocols
WB protocol for AP2B1 antibody 68349-1-IgDownload protocol
IF protocol for AP2B1 antibody 68349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols