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Validation Data Gallery

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  • Western Blot (WB) analysis of Hacat cells using AP1M2 Polyclonal antibody (10618-1-AP)

    WB analysis of Hacat cells using 10618-1-AP (same clone as 10618-1-PBS)

    WB result of AP1M2 antibody (10618-1-AP;1:500) with siRNA cell lysate. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse colon tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    WB analysis of mouse colon using 10618-1-AP (same clone as 10618-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using AP1M2 Polyclonal antibody (10618-1-AP)

    WB analysis of BxPC-3 using 10618-1-AP (same clone as 10618-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10618-1-AP (AP1M2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human pancreas cancer using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human bowen disease using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human bowen disease using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10618-1-AP (AP1M2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human kidney using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AP1M2 Polyclonal antibody (10618-1-AP)

    IHC staining of human kidney using 10618-1-AP (same clone as 10618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10618-1-AP (AP1M2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10618-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

AP1M2 Polyclonal antibody, PBS Only

AP1M2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10618-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10618-1-PBS targets AP1M2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0961

Product name: Recombinant human AP1M2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC005021

Sequence: MSASAVFILDVKGKPLISRNYKGDVAMSKIEHFMPLLVQREEEGALAPLLSHGQVHFLWIKHSNLYLVATTSKNANASLVYSFLYKTIEVFCEYFKELEEESIRDNFVIVYELLDELMDFGFPQTTDSKILQEYITQQSNKLETGKSRVPPTVTNAVSWRSEGIKYKKNEVFIDVIESVNLLVNANGSVLLSEIVGTIKLKVFLSGMPELRLGLNDRVLFELTGLSGSKNKSVELEDVKFHQCVRLSRFDNDRTISFIPPDGDFELMSYRLSTQVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANGVEISVPVPS

相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 2 subunit
Calculated molecular weight 48 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC005021
Gene Symbol AP1M2
Gene ID (NCBI) 10053
RRIDAB_2242895
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Q5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AP1M2 is a subunit of the adaptor complex AP-1. It belongs to the adaptor complexes medium subunit family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

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