• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis using 83613-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis of HEK-293 using 83613-6-RR

    WB result of AP1M1 antibody (83613-6-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1M1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using AP1M1 Recombinant antibody (83613-6-RR)

    WB analysis using 83613-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83613-6-RR (AP1M1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using AP1M1 Recombinant antibody (83613-6-RR)

    IHC staining of mouse skin using 83613-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83613-6-RR (AP1M1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using AP1M1 Recombinant antibody (83613-6-RR)

    FC experiment of HeLa using 83613-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of U-251 cells using AP1M1 Recombinant antibody (83613-6-RR)

    FC experiment of U-251 using 83613-6-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug AP1M1 Recombinant antibody (83613-6-RR, Clone:240553H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83613-6-RR

    Affinity and Kinetic Characterization of 83613-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83613-6-RR against Human AP1M1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

AP1M1 Recombinant monoclonal antibody

AP1M1 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83613-6-RR
Clone No.240553H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240553H3, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, AP 1 complex subunit mu 1, AP mu chain family member mu1A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, mouse testis tissue, PC-3 cells, K-562 cells, Jurkat cells, rat testis tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83613-6-RR targets AP1M1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2757

Product name: Recombinant human AP1M1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 164-423 aa of BC017469

Sequence: IKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPELRLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIESVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPGGKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQLRTQ

相同性解析による交差性が予測される生物種
Full Name adaptor-related protein complex 1, mu 1 subunit
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC017469
Gene Symbol AP1M1
Gene ID (NCBI) 8907
RRIDAB_3671226
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXS5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Protocols

Product Specific Protocols
FC protocol for AP1M1 antibody 83613-6-RRDownload protocol
IHC protocol for AP1M1 antibody 83613-6-RRDownload protocol
WB protocol for AP1M1 antibody 83613-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...